The conversion block can be put in the unsigned or signed conversion mode. Changing between unsigned/signed and single-ended/differential modes will corrupt data already in the pipeline.
Signed mode can be used as input mode for both differential and single-ended inputs, while unsigned mode is only available for the single-ended or internal input.
In unsigned mode the conversion range is from ground to the reference voltage. To be able to have zero-cross detection a ΔV is subtracted. The ΔV is approximately 0.05* VREF, so the ground level will be approximately 0.05 of the total value range (0.05 * 4095 with 12-bit resolution). This will also limit the maximum input voltage of 0.05* VREF, so the maximum input voltage is VREF - ΔV. This is illustrated in the figure below, "Unsigned and Signed Conversion Mode".
In signed mode the range is from negative to positive reference voltage, but the input voltage must be within GND and VREF. Figure "Unsigned and Signed Conversion Mode" shows the difference in conversion ranges.
The conversion mode is configured using the Conversion Mode bit (CONVMODE) in Control Register B (CTRLB).